Hypoxia is a known driver of tumourigenesis and a predictor of poor prognosis in many solid tumours. Our understanding of the role of hypoxia in tumour progression, however, largely comes from studies of relatively short hypoxia insult (usually ~24-48 hours). Physiologically, in solid tumours, tumour cells are exposed to hypoxic conditions for much longer period of time, so are the responses identified in the lab at 24h of hypoxia translatable to physiological conditions? In this project, we attempt to bridge this gap by studying how prolonged exposure to hypoxia impacts cancer cells, and impacts their aggressiveness